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KMID : 0861020120270030067
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Korea Journal of Herbology
2012 Volume.27 No. 3 p.67 ~ p.74
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Effect of Ssanghwa-tang Extract on Antioxidant and Anti-aging Enzyme Activities
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Park Ji-Young
Hwang Jae-Gyu
Yun Jong-Kuk
Han Kil-Hwan
Do Eun-Ju
Kim Sung-Ok
Kim Mi-Ryeo
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Abstract
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Objectives: The present study was designed to investigate effects of Ssanghwa-tang (Shu?nghu?-g?ng) on
oxidation/reduction reaction-related and aging-related enzymes in vitro.
Methods: We performed MTT assay, collagenase inhibition assay, elastase inhibition assay, tyrosinase inhibition
assay, DPPH free radical scavenging assay, SOD-like activity and xanthine oxidase (XO) inhibition assay.
Results: The 50% ethanol (EtOH) extract of Ssanghwa-tang (SHT) showed 55% inhibition of collagenase
activity, and 42% inhibition of elastase activity at 1 mg/ml concentration. Also it’s treatment showed 18%
inhibition of tyrosinase activity, to relate whitening effect, at the same dose of 50% ethanol extract of SHT.
Antioxidant activities were determined by DPPH radical scavenging, XO inhibiting activity and SOD-like
activity. These scavenging, XO-inhibiting and SOD-like activities were measured in 80%, 75%, and 28%
inhibitions, respectively, at a 1 mg/ml treated dose, compared to those of control. The inhibitory effects of 50%
EtOH extract on aging and oxidation-related enzyme activities were higher than those of water extract and
95% EtOH extract.
Conclusions: Taken together, our findings suggest that the SHT has potential and applicable benefits for
development of cosmetics to have anti-aging (anti-wrinkle and whitening) and anti-oxidation functions.
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KEYWORD
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Ssanghwa-tang, anti-aging, anti-oxidation, collagenase, elastase, tyrosinase
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